Advocating the Use of Loop-mediated Isothermal Amplification Assay to Improve the Rates of Diagnosis of Pulmonary Tuberculosis in Resource-constraint Settings
Saurabh R Shrivastava, Prateek S Shrivastava
Loop-mediated isothermal amplification, Tuberculosis, World Health Organization
Citation Information :
Shrivastava SR, Shrivastava PS. Advocating the Use of Loop-mediated Isothermal Amplification Assay to Improve the Rates of Diagnosis of Pulmonary Tuberculosis in Resource-constraint Settings. Indian J Respir Care 2023; 12 (2):199-200.
Tuberculosis (TB) continues to remain one of the top infectious diseases on a global scale. Acknowledging the gains observed, specific targets have been set as a part of the Sustainable Development Goals, and global leaders are hoping for significant improvement by 2030. In order to achieve this goal, an end TB strategy has been proposed, and great emphasis has been given toward enhancing case detection. Realizing the need, the World Health Organization (WHO) has given more attention toward the development and evaluation of new diagnostic tools and drug susceptibility testing. A molecular assay based on loop-mediated isothermal amplification (LAMP) has been developed to promote the detection of the Mycobacterium tuberculosis (M. tb) TB complex. This test can be easily performed at a peripheral level, requires minimal laboratory infrastructure and limited biosafety standards, is easy to use, and results are available in <1 hour. To conclude, there is an indispensable need to adopt newer methods to strengthen the diagnostic aspect of the disease. This need becomes even more important in resource-poor settings, and hence it is of utmost importance to implement cost-effective diagnostic tools to promote early detection and prompt initiation of treatment.
World Health Organization. Tuberculosis – Key Facts; 2022. Available from: https://www.who.int/en/news-room/fact-sheets/detail/tuberculosis [Last accessed on 2022 Dec 2].
The use of loop-mediated isothermal amplification (TB-LAMP) for the diagnosis of pulmonary tuberculosis: policy guidance. Geneva: World Health Organization 2016.
Ou X, Wang S, Dong H, et al. Multicenter evaluation of a real-time loop-mediated isothermal amplification (RealAmp) test for rapid diagnosis of Mycobacterium tuberculosis. J Microbiol Methods 2016;129:39–43. DOI: 10.1016/j.mimet.2016.07.008
Gray CM, Katamba A, Narang P, et al. Feasibility and operational performance of tuberculosis detection by loop-mediated isothermal amplification platform in decentralized settings: results from a multicenter study. J Clin Microbiol 2016;54(8):1984–1991. DOI: 10.1128/JCM.03036-15
Nliwasa M, MacPherson P, Chisala P, et al. The sensitivity and specificity of loop-mediated isothermal amplification (LAMP) assay for tuberculosis diagnosis in adults with chronic cough in Malawi. PLoS One 2016;11(5):e0155101. DOI: 10.1371/journal.pone.0155101
Nakiyingi L, Nakanwagi P, Briggs J, et al. Performance of loop-mediated isothermal amplification assay in the diagnosis of pulmonary tuberculosis in a high prevalence TB/HIV rural setting in Uganda. BMC Infect Dis 2018;18(1):87. DOI: 10.1186/s12879-018-2992-1
Shete PB, Farr K, Strnad L, et al. Diagnostic accuracy of TB-LAMP for pulmonary tuberculosis: a systematic review and meta-analysis. BMC Infect Dis 2019;19(1):268. DOI: 10.1186/s12879-019-3881-y
Singh P, Kanade S, Nataraj G. Sensitivity and specificity of loop-mediated isothermal amplification assay for diagnosis of extra-pulmonary tuberculosis: a cross-sectional study. European Resp J 2019;54:PA554. DOI: 10.1183/13993003.congress-2019.PA554