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VOLUME 13 , ISSUE 4 ( October-December, 2024 ) > List of Articles

Original Article

Role of Hydroxyl Radical in the Progression of Chronic Obstructive Pulmonary Disease

Vinay Pathak, Navneet Kumar, Pradeep Nirala

Keywords : Chronic obstructive pulmonary disease, Deoxyribonucleic acid oxidation, Hydroxyl radical, Lipid peroxidation, Protein oxidation, Singlet oxygen, Superoxide anion radical

Citation Information : Pathak V, Kumar N, Nirala P. Role of Hydroxyl Radical in the Progression of Chronic Obstructive Pulmonary Disease. Indian J Respir Care 2024; 13 (4):226-232.

DOI: 10.5005/jp-journals-11010-1141

License: CC BY-NC 4.0

Published Online: 17-02-2025

Copyright Statement:  Copyright © 2024; The Author(s).


Abstract

Aim and background: Chronic obstructive pulmonary disease (COPD) is one of the primary factors contributing to death globally each year. Reactive oxygen species (ROS) are recognized to aid in the development of this disease, but which ROS contributes the most is not clearly understood yet. This research sought to look into which ROS contributes the most to the progression of COPD. Materials and methods: To achieve this aim, we assessed the quantities of the three major ROS contributing to human diseases, which are superoxide anion radical (O2•−), hydroxyl radical (•OH), and singlet oxygen (1O2) in COPD patients as well as healthy individuals by electron spin resonance (ESR) spectroscopy. We also measured the quantities of markers of biomolecule (lipid, protein, DNA) oxidation, that is, malondialdehyde, protein carbonyl groups, and 8-hydroxy-2-deoxyguanosine, in COPD patients and healthy controls using respective ELISA kits. Results: The level of O2•− in control samples was 3460 ± 170 r.u., while in patient samples it was 7842 ± 166 r.u. The •OH level was found to be 8460 ± 850 r.u. and 16560 ± 1425 r.u. in the control and patient samples, respectively. The 1O2 level in controls was 2728 ± 263 r.u., and in patients, it was 3728 ± 249 r.u. The level of MDA was 203.20 ± 9.73 pg/mL and 434.00 ± 39.11 pg/mL in controls and patients, respectively. The levels of protein carbonyl group formation and 8-OHdG were 6.98 ± 0.84 mmol/mL and 15.84 ± 2.06 ng/mL in controls, while 16.67 ± 1.44 mmol/mL and 33.66 ± 1.92 ng/mL in patients, respectively. Conclusion: The outcomes of our research point out that hydroxyl radical is the major ROS formed in COPD patients, contributing to the progression of the disease by mainly oxidizing DNA.

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